摘要： This work addresses the use of a thin-film short wave ultraviolet radiation (UV-C) reactor and its combination with ultra-high pressure homogenization (UHPH) for the inactivation of Talaromyces macrosporus (CBS 130.89) and Neosartorya spinosa (CBS 586.90), fungal species that can cause food spoilage in products made of fruits. Ascospores of these microorganisms were inoculated in phosphate buffer solution (PBS) and clarified apple juice at a concentration between 5 and 6 Log10 spores/mL before treatments. Ascospores of both microorganisms were not detected in PBS after UV-C treatments even at the lowest assayed dose (1.8 J/mL), but in apple juice T. macrosporus showed to be significantly more UV-C resistant than N. spinosa. A UV-C dose of 21.5 J/mL in a single pass achieved decimal reductions of 2.15 Log10 and 5.4 Log10 respectively. Decimal reductions of both strains significantly increased in many cases when the same UV-C dose was applied in 2 or 3 passes throughout the UV-C reactor achieving T. macrosporus a maximum reduction of 3.88 Log10 after a UV-C dose of 21.5 J/mL applied in 3 passes. UHPH treatments at 100 and 200 MPa were ineffective in inactivating ascospores of both microorganisms, However, UHPH left ascospores more vulnerable to UV-C resulting in a synergistic effect achieving a maximum reduction of 3.6 Log10 on T. macrosporus after a combined treatment of UHPH at 200 MPa and a single pass of UV-C at 21.5 J/mL. The use of scanning electron microscopy revealed that only UHPH caused small changes in the cell wall structure of ascospores, but that might have left cells better exposed to UV-C.