研究目的
To determine the feasibility of monitoring flap vascular patency through noninvasive microcirculation monitoring using SDF.
研究成果
Microscan SDF is a feasible method to assess the perfusion status in cutaneous flaps and it should be considered as a valid alternative to other monitoring tools. It allows earlier detection of impaired microcirculation in both venous and total vascular occlusions compared to other methods.
研究不足
The study was conducted on a small sample size of five volunteers. The method requires further validation in clinical practice with real microsurgical flaps.
1：Experimental Design and Method Selection:
An experimental study was performed in five volunteers to simulate a radial forearm flap in both upper extremities. Cutaneous microcirculation assessment devices (SDF and NIRS) were used and compared with clinical and Doppler evaluation. Conditions of venous occlusion (VO) and total vascular occlusion (TVO) were mimicked using a cuff.
2：Sample Selection and Data Sources:
Five healthy volunteers aged 21 to 33 years were recruited. A perforator vessel inside the design was identified and marked with a handheld Doppler.
3：List of Experimental Equipment and Materials:
Sidestream dark field (Microscan SDF), near infrared spectroscopy (NIRS), cuff (Welch Allyn DS65), handheld sound Doppler SonoTrax Lite?, infrared clinical Beurer JFT-90? Thermometer.
4：Experimental Procedures and Operational Workflow:
Venous and total vascular occlusions were achieved using a cuff for 180 seconds, and changes were monitored with each method. Clinical evaluation was performed using temperature, color, capillary refill. The presence or absence of flow in the perforator vessel was monitored using a handheld sound Doppler.
5：Data Analysis Methods:
The Microvascular Flow Index (MFI) was used for quantitative analysis of microcirculatory flow. Statistical analysis was performed using the Mann-Whitney/Wilcoxon test for quantitative variables and χ2 for categorical variables.
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