研究目的
To present a new FTIR-based micro?uidic system for Glucose, Fructose and Sucrose detection, improving the uniformity of sample distribution in the analyzed area versus conventional systems.
研究成果
The developed micro?uidic system significantly improves the uniformity of sugar distribution in the analyzed area, reducing measurement errors. The system's ability to accurately identify and quantify sugar types in aqueous solutions was demonstrated, with a maximum quanti?cation error of 7.9%. The quadratic ?t approach provided better concentration estimation than linear approximation.
研究不足
The study assumes the liquid solution has been pretreated to contain only one type of sugar, limiting the results to homogeneous solutions. The achieved limit of detection is related to the commercial microscope-FTIR system used.
1：Experimental Design and Method Selection:
The study employs a pseudo-continuous ?ow system coupled to a microscope-FTIR instrument for sugar detection. The methodology focuses on recording absorption spectra in the Mid-IR region (700–1000 cm?1).
2：1).
Sample Selection and Data Sources:
2. Sample Selection and Data Sources: Glucose, Fructose, and Sucrose samples were prepared in different concentrations using deionized water as a solvent.
3：List of Experimental Equipment and Materials:
Includes a microscope-FTIR spectrometer (Spotlight 400 FT-IR Imaging System, Perkinelmer), micro?uidic chip with a heating system, and a pumping subsystem.
4：Experimental Procedures and Operational Workflow:
Samples were injected into the analysis area, heated to evaporate the solvent, and cooled before recording absorption spectra. The process was repeated for different concentrations to verify repeatability.
5：Data Analysis Methods:
Absorption spectra were analyzed to identify and quantify sugar types based on their characteristic peaks. The system's performance was evaluated by comparing the uniformity of sample distribution and measurement errors with conventional methods.
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