研究目的
To develop a multiplex detection platform for the identification of semen and vaginal fluid (VF) using a small amount of sample, which is important in cases of sexual crime.
研究成果
The developed HSV-ID chip demonstrated accurate selectivity with no cross-reactivity between the semen and VF samples. The LOD of the HSV-ID chip was 0.06 μg/mL for semen and 0.005 μg/mL for VF, which was 10 times better than that of the commercially available RSID-Semen kit.
研究不足
The study focused on the identification of semen and vaginal fluid (VF) only. The validation study using various criminal samples and the extension of detectable body fluids including saliva, blood, and urine are subjects of ongoing research.
1：Experimental Design and Method Selection:
The study proposed a multiplex detection platform using an Ag vertical nanorod metal enhanced fluorescence (MEF) substrate. The Ag nanorod MEF substrate was fabricated by glancing angle deposition (GLAD), and amino functionalization was conducted to improve binding ability.
2：Sample Selection and Data Sources:
Semen and vaginal fluid (VF) samples were used for the identification.
3：List of Experimental Equipment and Materials:
Ag nanorod MEF substrate, semenogelin-2 antibody, anti-17 beta estradiol antibody, Dylight? 633, microarray scanner (GenePix 4000B).
4：Experimental Procedures and Operational Workflow:
The fabricated HSV-ID chip was attached to a 16-chamber aluminum jig for the identification of body fluid samples. The Dylight-conjugated detection sample was injected into a single chamber and incubated for antibody and biomarker binding. After incubation, the chamber was washed, and the fluorescence signal was measured.
5：Data Analysis Methods:
The fluorescence signal was measured using a microarray scanner with an excitation wavelength of 635 nm.
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