摘要： We propose a high-sensitive Sagnac-interferometer biosensor based on the Vernier effect (VE) with a high-birefringence micro?ber. The sensitivity enhancement is achieved by utilizing two cascaded Sagnac interferometers. One of the two interference loops consists of a panda polarization-maintaining ?ber as a ?lter, whilst the other is comprised of high-birefringent micro?ber coated Graphene oxide (GO) as a sensing channel. We theoretically analyzed the sensitivity of the sensor and veri?ed it with experiments. The results of the simulation show that the refractive index sensitivity is more than ?ve times that of the ?ber sensor based on a single Sagnac loop. The sensitivity of the refractive index in the experiments can reach 2429 nm/refractive index unit (RIU), which is basically in accordance with the simulation. We also use electrostatic adsorption to coat GO on the surface of the sensing channel. GO is employed to adsorb bovine serum albumin (BSA) molecules to achieve the desired detection results, which has good biocompatibility and large speci?c surface area. The sensitivity to detect BSA can reach 9.097 nm/(mg×mL?1).