研究目的
Introducing a simple, rapid, sensitive and selective label-free assay for Cytochrome c (Cyt c) detection based on an interaction between nucleic acid aptamer biomolecules and surfaces of Carbon Dots (CDs).
研究成果
A sensitive aptamer-based assay for the detection of Cyt c was developed, showing high sensitivity with a detection limit of 25.90 nM and a wide linear range from 40 nM to 240 nM. The assay is highly selective and can be used for the detection of Cyt c in real samples.
研究不足
The method's sensitivity and selectivity are high, but the detection limit of 25.90 nM may not be sufficient for detecting very low concentrations of Cyt c in some biological samples.
1：Experimental Design and Method Selection:
The method is based on the interaction of carbon dots and aptamer molecules. CDs were prepared by directly pyrolysis of citric acid (CA).
2：Sample Selection and Data Sources:
Cyt c-aptamer was synthesized by Pishgam Biotech Co. (Tehran, Iran).
3：List of Experimental Equipment and Materials:
Perkin-Elmer LS50 luminescence spectrometer, Fourier-transform infrared spectrometer (PerkinElmer, USA), Transmission electron microscope (TEM).
4：Experimental Procedures and Operational Workflow:
CDs and Cyt c-aptamer were mixed and incubated to react at 37 °C for 1 h. Then, Cyt c was added, and the mixture was incubated at 37 °C for 15 min before fluorescence measurements.
5：Data Analysis Methods:
The fluorescence intensity was measured, and the plot of ΔF versus the logarithm concentrations of Cyt c was used for quantification.
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