研究目的
To determine whether nonlinear optical microscopy can be used to identify gastric intestinal metaplasia and to further differentiate the subtypes of intestinal metaplasia without the need for exogenous contrast agents.
研究成果
Nonlinear optical microscopy has the potential to directly differentiate gastric intestinal metaplasia from normal tissues and to further classify the subtypes of intestinal metaplasia without the need for labels. This technique could serve as a valuable diagnostic tool in the future, especially if integrated into endoscopic procedures.
研究不足
The study is limited by the small sample size of five biopsy tissues. Additionally, the technique's potential for real-time in vivo application during endoscopy requires further development and validation.
1：Experimental Design and Method Selection:
Nonlinear optical microscopy using two-photon excited fluorescence (TPEF) combined with second harmonic generation (SHG) was employed to investigate gastric mucosal intestinal metaplasia.
2：Sample Selection and Data Sources:
Five biopsy tissues of gastric intestinal metaplasia and normal gastric mucosal tissues were collected for comparison.
3：List of Experimental Equipment and Materials:
An inverted microscope (LSM 510 META, Zeiss, Germany) combined with a mode-locked femtosecond Ti:sapphire laser (Mira 900-F, Coherent, Inc., USA) was used.
4：Experimental Procedures and Operational Workflow:
Serial sections were cut at 10 μm by a cryostat microtome, and nonlinear optical imaging was performed.
5：Data Analysis Methods:
Nuclear areas from normal gastric mucosa and intestinal metaplasia subtypes were measured, and statistical analyses were performed using IBM SPSS Statistics 21.
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