研究目的
Investigating the resonance energy transfer in genetically engineered polypeptides and its impact on fluorescence intensity.
研究成果
The study demonstrates that energy transfer in genetically engineered polypeptides can result in unanticipated fluorescence intensity increases, even at distances beyond the classical F?rster radius. This suggests strong electronic coupling between donor chromophores and potential applications in light-harvesting systems.
研究不足
The study is limited by the relatively low tryptophan extinction coefficient and potential aggregation effects on fluorescence measurements.
1：Experimental Design and Method Selection:
Genetically engineered polypeptides were designed to form β-sheet conformations with varying lengths to study resonance energy transfer.
2：Sample Selection and Data Sources:
Peptides KWEW4-C1, KWEW8-C1, KWEW12-C1, and KWEW16-C1 were prepared and their folding verified by CD and deep UV resonance Raman experiments.
3：List of Experimental Equipment and Materials:
Circular dichroism spectrometer, deep UV resonance Raman spectrometer, fluorescence spectrometer.
4：Experimental Procedures and Operational Workflow:
Peptides were irradiated at 280 nm, and fluorescence intensity was measured. Control experiments with free tryptophan and DACM were conducted.
5：Data Analysis Methods:
Fluorescence lifetimes and quantum yields were calculated to assess energy transfer efficiency.
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