研究目的
To determine the antioxidant properties of several C. japonica cultivars leaves and to propose an easy, costless, large-scale and effective spectroscopic-based method to predict such properties.
研究成果
The study successfully determined the antioxidant profile of C. japonica cultivars leaves and proposed a rapid, low-cost spectroscopic-based method for its determination. NIRS provided more satisfactory predictions than MIRS, with higher coefficients of determination and lower errors. The developed PLS models are good for calibration and quantification purposes.
研究不足
The study acknowledges fluctuations in the antioxidant profile across different trees of the same cultivar, possibly due to geographic region and metabolic state. The soil composition could also impact the results. The study suggests further research to identify bioactive compounds in C. japonica cultivars.
1:Experimental Design and Method Selection:
The study involved determining the antioxidant profile (TPC, TFC, and TAC) of 31 C. japonica cultivars leaves using standard analytical methods and assessing these profiles by near- and mid-infrared spectroscopy.
2:Sample Selection and Data Sources:
Leaves from 58 C. japonica plants, including 31 different cultivars, were collected from two distinct locations.
3:List of Experimental Equipment and Materials:
Chemicals of analytical reagent grade, Fourier transform near-infrared spectrometer (FTLA 2000, ABB, Canada), Fourier transform PerkinElmer Spectrum BX FTIR System spectrophotometer (USA), and others.
4:Experimental Procedures and Operational Workflow:
Leaves were air-dried, ground into fine powder, and extracted. Spectra were acquired using NIRS and MIRS techniques.
5:Data Analysis Methods:
Chemometric methods including PCA for outlier detection and PLS regression for modelling TPC, TFC, and TAC were applied.
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