研究目的
Investigating the development of a sensitive fluorescence sensor for point-of-care detection of trypsin using glutathione-stabilized gold nanoclusters.
研究成果
The developed fluorescence sensing platform based on AuNCs and Cyt c provides a sensitive, selective, and convenient method for the detection of TRY. The system's applicability in biological samples and the development of test strips for visual detection highlight its potential for point-of-care applications.
研究不足
The study focuses on the detection of TRY and may not be directly applicable to other biomarkers without further modification. The sensitivity and specificity of the sensor in complex biological matrices need further validation.
1：Experimental Design and Method Selection:
The study utilized a fluorescence nanosensor based on gold nanoclusters (AuNCs) for the detection of trypsin (TRY). The fluorescence intensity of AuNCs was quenched by cytochrome c (Cyt c) via electron transfer mechanism, and TRY catalyzed the hydrolysis of Cyt c, leading to fluorescence recovery.
2：Sample Selection and Data Sources:
Biological samples including human blood and urine were used for the detection of TRY.
3：List of Experimental Equipment and Materials:
Hydrogen tetrachloroaurate (III) hydrate (HAuCl4? xH2O), L-glutathione, TRY (180 U mg-1), and other chemicals were obtained from Sigma-Aldrich Corporation. AuNCs were synthesized using a one-pot synthetic method.
4：Experimental Procedures and Operational Workflow:
AuNCs were synthesized and characterized. The fluorescence quenching effect of Cyt c on AuNCs was studied, and the detection procedure for TRY activity was optimized. Test strips were developed for visual detection of TRY.
5：Data Analysis Methods:
The fluorescence spectra were measured using a spectrofluorophotometer. ImageJ software was used for processing color values for quantitative detection.
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