研究目的
Investigating the method to perform super-resolution label-free optical microscopy by means of the saturation of the transient absorption process.
研究成果
The saturated pump-probe technique allows achieving a resolution that could be better than 100 nm while using wavelengths longer than 1000 nm, enabling three-dimensional super-resolution imaging without the addition of any fluorescent label.
研究不足
The technique is specific for the molecular transition of the matter under investigation and requires samples that comprise a known saturable absorption transition. The resolution depends on the power of the saturating pump beam.
1：Experimental Design and Method Selection:
The study employs a novel and easy-to-implement setup based on a commercial confocal microscope, utilizing near-infrared femtosecond pulsed laser beams and saturation of the transient absorption for super-resolution imaging.
2：Sample Selection and Data Sources:
Single layer graphene (SLG) deposited on a glass surface via chemical vapour deposition (CVD) is used to validate the performance of the setup.
3：List of Experimental Equipment and Materials:
Includes a Nikon Eclipse FN1 upright microscope body, a Nikon C2 scan head, a Chameleon Ultra II laser source, a Compact OPO, an electro-optical modulator, beam expanders and collimators, a vortex phase plate, and a 100×
4：3 NA oil objective lens. Experimental Procedures and Operational Workflow:
The pump beam is modulated at 2 MHz, and the saturating pump is applied slightly delayed in time to saturate the signal at the periphery of the focal spot. The probe signal is collected in transmission with a photodiode.
5：Data Analysis Methods:
The demodulated pump-probe signal is acquired by the Nikon controller and analyzed with FIJI software.
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Nikon Eclipse FN1 upright microscope
FN1
Nikon Instruments
Microscope body for optical imaging
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Nikon C2 scan head
C2
Nikon Instruments
Customized scan head for free-space NIR laser coupling
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Chameleon Ultra II
Ultra II
Coherent Inc.
Laser source for pumping the OPO
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Compact OPO
Compact OPO
Coherent Inc.
Optical parametric oscillator for generating pump and probe beams
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Vortex phase plate
VPP 1a
RPC photonics
Creating the doughnut shape for the saturating pump beam
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100× 1.3 NA oil objective lens
100× 1.3 NA
Nikon Instruments
Focusing the beams for imaging
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Photodiode
DET36A
Thorlabs Inc.
Collecting the probe signal in transmission
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Digital delay generator
DG645
Stanford Research Systems
Controlling and generating the electronic synchronization signal
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Electro-optical modulator
LM 0202
Qioptiq
Modulating the pump beam at 2 MHz
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Lock-in amplifier
HF2LI
Zurich Instrument
Demodulating the pump-probe signal
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