研究目的
To develop new types of BODIPY-based fluorescent probes capable of cysteine (Cys)/homocysteine (Hcy) sensing with high selectivity over other amino acids and to study the influence of different electronegativity substituents on these probes.
研究成果
The study successfully developed three novel BODIPY-based fluorescent probes for the selective detection of Cys and Hcy. The electron-withdrawing group on probe 1 was found to accelerate its response to Cys/Hcy, making it suitable for imaging these biothiols in living cells. This research contributes to the field of biothiol detection with potential applications in disease diagnosis and monitoring.
研究不足
The study focused on the detection of Cys and Hcy, with less emphasis on distinguishing between these two biothiols and GSH. The probes' performance in complex biological samples beyond controlled laboratory conditions was not extensively explored.
1：Experimental Design and Method Selection:
The study involved the synthesis of three BODIPY-based fluorescent probes with different electronegativity substituents to investigate their sensing capabilities for Cys/Hcy.
2：Sample Selection and Data Sources:
The probes were tested against various amino acids and biologically related ions to evaluate selectivity and sensitivity.
3：List of Experimental Equipment and Materials:
Instruments included a Varian Model Mercury 400 MHz spectrometer for NMR, a Bruker Reflex MALDI for mass spectrometry, and a FluoroSENS spectrophotometer for fluorescence spectra.
4：Experimental Procedures and Operational Workflow:
The synthesis of probes involved multiple steps including bromination, azide substitution, and dehydrobromination, followed by testing their fluorescence response to Cys/Hcy.
5：Data Analysis Methods:
Fluorescence intensity changes were measured and analyzed to determine the detection limits and selectivity of the probes.
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