摘要： Our previous work has demonstrated that α-linolenic acid (ALA)-loaded oil-in-water (O/W) microemulsion could enhance ALA antioxidant capacity. Meanwhile, we also observed that synthesized microemulsion itself had ?uorescence. In this work, we have prepared a multiple water-in-oil-in-water (W/O/W) microemulsion to further enhance ALA antioxidant capacity and activate this delivery carrier application potential with a free label. The compositions of primary water-in-oil (W/O) microemulsion were obtained using pseudo-ternary phase diagrams, and then W/O/W microemulsion was prepared adopting the “two-step heterotherm method”. The conductivity of W/O/W microemulsion was measured to lie between 250.0 and 350.0 μs/cm. The spherical droplets with a mean particle diameter of 10.0?20.0 nm were con?rmed by transmission electron microscopy and dynamic light scattering. Nuclear magnetic resonance con?rmed that ALA di?used to the multiple water?oily interface simultaneously. In addition, the in vitro release and antioxidant capacity measurements of ALA-loaded W/O/W microemulsion concluded the sustained-release e?ect and excellent antioxidant capacity. The ?uorescent intensity of W/O/W microemulsion was markedly increased in comparison to O/W microemulsion. The synthesized microemulsion could lead to important applications and have advantages of a label-free ?uorescent carrier for optical imaging purposes.