研究目的
Investigating the phase separation dynamics of water and 2-butoxyethanol mixtures using time-resolved structured illumination microscopy and fluorescence spectroscopy to understand the early and late stages of spinodal decomposition.
研究成果
The study successfully applied time-resolved SIM and fluorescence spectroscopy to observe the phase separation dynamics of water/2BE mixtures. It revealed that the early stage involves the formation of water-poor 2BE aggregates, with the number density of these aggregates increasing over time. The late stage, characterized by the growth of periodic structures, begins after approximately 10 μs. This provides a new understanding of the spinodal decomposition process in binary liquid mixtures.
研究不足
The study is limited by the temporal and spatial resolution of the techniques used, particularly in capturing the very early stages of phase separation. The interpretation of fluorescence spectral shifts relies on the assumption that the probe accurately reflects the microenvironmental changes during phase separation.
1：Experimental Design and Method Selection:
The study utilized time-resolved structured illumination microscopy (SIM) and fluorescence spectroscopy to observe phase separation dynamics. A nanosecond temperature jump (T-jump) method was employed to induce phase separation.
2：Sample Selection and Data Sources:
Water/2-butoxyethanol mixtures with various molar fractions were used, with a hydrophobic fluorescent probe (BPDI) to visualize the organic phase.
3：List of Experimental Equipment and Materials:
Equipment included a nanosecond NIR laser for T-jump, a fluorescence spectrometer, a spectrograph with a cooled CCD camera, and a spatial light modulator for SIM.
4：Experimental Procedures and Operational Workflow:
The T-jump was induced by nanosecond NIR pulses, and fluorescence images and spectra were captured at various delay times. SIM was used to achieve super-resolution imaging.
5：Data Analysis Methods:
Fluorescence spectra and images were analyzed to determine spectral shifts and characteristic lengths of phase-separating domains. Power spectra from SIM and light scattering were used to analyze domain sizes and growth dynamics.
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