研究目的
Demonstrating an ex-vivo device based on a silica optical fibre for monitoring of locally variable cytokine IL-6 concentrations using a sandwich immunoassay scheme.
研究成果
The fabricated optical fibre based cytokine device is sensitive and specific for IL-6 detection, with potential for monitoring spatially varying concentrations of cell-secreting products and development into point-of-care devices for multiple health conditions.
研究不足
The study focuses on IL-6 detection and may require adaptation for other cytokines. The ex-vivo application needs further development for in-vivo use.
1：Experimental Design and Method Selection:
The sensing interface was fabricated by modifying an exposed optical fibre with gold nanoparticles and functionalizing it with IL-6 capture antibody. A sandwich immunoassay scheme was used for detection.
2：Sample Selection and Data Sources:
The device was tested with cytokine IL-6 in PBS solution and with IL-6 secreted by BV2 cells following liposaccharide stimulation.
3：List of Experimental Equipment and Materials:
Silica optical fibre, gold nanoparticles, anti-IL-6 capture antibody, fluorescently labelled magnetic nanoparticles, BV2 cells, liposaccharide.
4：Experimental Procedures and Operational Workflow:
The fibre was modified stepwise, incubated with cytokine solution, and then exposed to detection antibody loaded on fluorescently labelled magnetic nanoparticles. Fluorescence intensity was measured to quantify cytokine concentration.
5：Data Analysis Methods:
Fluorescence spectra and confocal microscopy images were analyzed to confirm successful attachment of detection antibody and quantify IL-6 concentration.
独家科研数据包，助您复现前沿成果，加速创新突破
获取完整内容
