研究目的
To develop a fluorescent 'turn-on' probe for alkaline phosphatase (ALP) detection with aggregation-induced emission (AIE) feature for application in aqueous media and living cells.
研究成果
The study successfully developed an AIE-active fluorescent probe for ALP detection with good water solubility and longer-wavelength emission. The probe demonstrated high sensitivity and selectivity for ALP in aqueous solution and was effectively applied in imaging ALP in live cells. This work provides insights into designing detection strategies for other enzymes.
研究不足
The study focuses on the detection of ALP and its application in cell imaging, but the probe's performance in more complex biological systems or in vivo was not explored. The specificity of the probe towards ALP over other enzymes was demonstrated, but potential interference in complex biological samples was not fully investigated.
1：Experimental Design and Method Selection:
The probe TPE-CN-pho was synthesized with a D-A-D structure incorporating a cyano group and a phosphate group. The design rationale was to achieve high conjugation degree and good water solubility for longer-wavelength emission.
2：Sample Selection and Data Sources:
The probe's response to ALP was tested in Tris buffer (10 mM, pH
3：0). Cell imaging was performed using HeLa, L929, and HepG2 cells. List of Experimental Equipment and Materials:
Instruments included a Bruker Avance 600 MHz NMR spectrometer, Hitachi F4600 fluorescence spectrometer, and Olympus IX 71 fluorescence microscope. Materials included ALP from bovine intestinal mucosa, EDTA, and various chemical reagents.
4：Experimental Procedures and Operational Workflow:
The probe's fluorescence response to ALP was measured after incubation at 37°C. Cell imaging involved incubating cells with the probe and observing fluorescence.
5：Data Analysis Methods:
Fluorescence spectra were recorded, and particle size analysis was performed using dynamic light scattering (DLS).
独家科研数据包，助您复现前沿成果，加速创新突破
获取完整内容
