研究目的
Investigating the fabrication of silver seeds and nanoparticles on core-shell Ag@SiO2 nanohybrids for combined photothermal therapy and bioimaging.
研究成果
The study successfully fabricated two types of core-shell nanohybrids with potential applications in bioimaging and photothermal therapy. Ag@SiO2@Agseed exhibited higher photothermal conversion efficiency and caused greater cell death upon light exposure compared to Ag@SiO2@AgNPs. These findings suggest that the fabricated nanohybrids, especially Ag@SiO2@Agseed, could be promising candidates for phototherapy-based biomedical applications.
研究不足
The study focuses on in vitro applications, and the biocompatibility and photothermal effects in vivo remain to be explored. The photothermal therapy efficacy is dependent on the wavelength of light used, and the study does not explore the effects of other wavelengths.
1：Experimental Design and Method Selection:
The study involved the fabrication of two core-shell nanohybrids using the Stober method, characterized by different morphologies (Ag@SiO2@Agseed and Ag@SiO2@AgNPs). The photothermal conversion efficiency and biocompatibility of these nanohybrids were evaluated.
2：Sample Selection and Data Sources:
HeLa cells were used for in vitro MTT assays and bioimaging to assess the nanohybrids' biocompatibility and photothermal therapy efficacy.
3：List of Experimental Equipment and Materials:
Materials included silver nitrate (AgNO3), polyvinylpyrrolidone (PVP), tetraethyl orthosilicate (TEOS), sodium borohydride (NaBH4), ammonia, and others. Equipment included UV-Vis spectrophotometer, fluorescence spectrometer, FE-SEM, TEM, and STEM.
4：Experimental Procedures and Operational Workflow:
The synthesis of nanohybrids involved solvothermal synthesis and the Stober method. Photothermal efficiency was tested by exposing nanohybrid solutions to 400 nm laser light and measuring temperature elevation. Cell viability and apoptosis were assessed using MTT assays and fluorescence microscopy.
5：Data Analysis Methods:
UV-Vis and fluorescence spectra were analyzed to characterize the nanohybrids. Photothermal conversion efficiency was evaluated based on temperature elevation. Cell viability and apoptosis were quantified using fluorescence microscopy and MTT assay results.
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