研究目的
To evaluate the feasibility of using re?ectance spectroscopy to monitor arsenic in rice plants and to assess the sensitivity of various vegetative indices (VIs) to arsenic levels.
研究成果
The study demonstrates that reflectance spectroscopy can be used to monitor arsenic levels in rice plants. Vegetative indices based on leaf spectra were strongly related with arsenic levels, but at canopy scale, soil background reflectance and canopy cover affected the performance of VIs. The combined index, TCARI/OSAVI, showed higher sensitivity to arsenic levels and better resistance to soil backgrounds and LAI.
研究不足
The study is based on controlled conditions in a growth chamber and simulation results for canopy reflectance. Field conditions with abiotic and biotic factors may confound with variations in reflectance characteristics.
1:Experimental Design and Method Selection:
Hydroponic growth chamber experiment with four arsenic levels induced in rice plants. Reflectance spectra of leaves were acquired over visible and infrared wavelengths. Canopy reflectance was simulated using the SAIL model for various soil moisture conditions and leaf area indices (LAI).
2:Sample Selection and Data Sources:
Rice seeds of the Jefferson cultivar were germinated and grown under controlled conditions with four arsenic treatments.
3:List of Experimental Equipment and Materials:
Spectroradiometer (ASD FieldSpec?), integrating sphere (LiCor LI-1800), spectrophotometer (Perkin-Elmer), inductively coupled plasma atomic emission spectrometry (ICP-AES).
4:Experimental Procedures and Operational Workflow:
Seedlings were transferred to polyethylene beakers with arsenic treatments. Reflectance measurements were taken at mid- to late-tillering stage. Leaf chlorophyll content and arsenic concentration were measured.
5:Data Analysis Methods:
Regression analysis and ANOVA were used to evaluate the performance of vegetative indices at leaf and canopy scales.
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