摘要： The present paper reports a new method for the quanti?cation of the Ni2+-capacity of an immobilized metal af?nity chromatography (IMAC) material using near-infrared spectroscopy (NIRS). Conventional analyses using UV absorption spectroscopy or atomic absorption spectrometry (AAS) need to dissolve the silica-based metal chelate sorbent as sample pretreatment. In the ?rst step, those methods were validated on the basis of an ideal homogenous NiSO4-solution and unveiled that UV with an intermediate precision of 2.6% relative standard deviation (RSD) had an advantage over AAS with an intermediate precision of 6.5% RSD. Therefore, UV analysis was chosen as reference method for the newly established NIRS model which has the advantage of being able to measure the material directly in diffuse re?ection mode. Partial least squares regression (PLSR) analysis was used as multivariate data analysis tool for quanti?cation. The best PLSR result obtained was: coef?cient of determination (R2) = 0.88, factor = 2, root mean square error of prediction (RMSEP) = 22 μmol/g (test-set validation) or 7.5% RSDPLSR. Validation of the Ni2+-capacity using UV absorption spectroscopy resulted in an intermediate precision of ±18 μmol/g or 5.0% RSD. Therefore, NIRS provides a fast alternative analysis method without the need of sample preparation.