研究目的
This study addresses the synthesis and application of photoactive proton transfer compounds based on the benzazolic core containing the azide group for the detection of hydrogen sulfide (H2S) in solution and biological media, utilizing a fluorescence off–on response mechanism.
研究成果
The synthesized azide derivatives exhibited significant changes in their photophysical properties in the presence of NaHS, enabling their application as optical sensors for H2S. The fluorescence off–on response mechanism was successfully demonstrated in solution and biological media. Theoretical calculations provided insights into the photophysics of the azide derivatives, explaining the observed fluorescence quenching. The compounds showed potential for probing H2S in fixed cells, suggesting further studies in living systems.
研究不足
The study primarily focused on the synthesis and photophysical characterization of azide derivatives for H2S detection. The application in living cells was not extensively explored, and further experiments are required to evaluate the probes' efficacy in vivo.
1：Experimental Design and Method Selection:
The study involved the synthesis of azide derivatives based on the benzazolic core, followed by photophysical characterization and application as H2S sensors. The methodology included the reduction of azides to amines using NaHS to mimic H2S, resulting in a fluorescence off–on response.
2：Sample Selection and Data Sources:
The compounds were synthesized and characterized using various spectroscopic techniques. The photophysical response to NaHS was evaluated in ethanol/PBS buffer solution.
3：List of Experimental Equipment and Materials:
UV-Vis spectrophotometer, spectrofluorometer, time-resolved fluorescence spectrophotometer, and fluorescence microscopy were used.
4：Experimental Procedures and Operational Workflow:
The synthesis of azide derivatives, their photophysical characterization, and application as H2S sensors were performed. The reaction kinetics and cell staining experiments were also conducted.
5：Data Analysis Methods:
The photophysical data were analyzed using UV-Vis and fluorescence spectroscopy. Theoretical calculations were performed using DFT and TD-DFT methods.
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