摘要： The development of nanotechnology has endowed the light-matter interaction with more degrees of freedom, and made the manipulation of optical phenomena more precise and arbitrary. Fano resonance is a type of resonant scattering phenomenon with asymmetric line profile, which was firstly studied in atomic physics and then introduced to photonics and plasmonics. This phenomenon is due to the interference between a discrete bound state and a continuum state, thus a two-body or few-body system is needed to provide different types of states that are necessary in this phenomenon. Artificial metamaterial is a very good candidate to generate Fano resonances because of its designable configuration. In this paper, a new type of structure—3D folding metamaterial—is introduced for Fano resonance generation and application. The structure, fabricated by focused-ion-beam based folding technique, is composed of planar and out-of-plane parts, which are essential for the excitation of the discrete bound states and continuum states. The intensity, frequency and quality factor of the Fano resonances can be modulated by the configuration of the 3D folding structures (shape, size, inclined angle, etc.), thus enlarges its application potential such as index sensing and surface enhanced Raman scattering.