研究目的
To enhance the antitumor efficiency by combining photodynamic therapy (PDT) with photothermal therapy (PTT) for targeted colorectal cancer therapy using hyaluronic acid-decorated polydopamine nanoparticles with conjugated chlorin e6 (HA–PDA–Ce6).
研究成果
HA–PDA–Ce6 nanoparticles showed promising results for targeted PDT and PTT treatment in colorectal cancer therapy, with enhanced tumor accumulation, deeper penetration, and significant tumor growth inhibition.
研究不足
The study primarily focuses on colorectal cancer and the specific combination of PDT and PTT using HA–PDA–Ce6. The in vivo fate of HA–PDA–Ce6 after peritumoral injection and detailed mechanism of degradation need further study.
1：Experimental Design and Method Selection:
The study involved the synthesis of HA–PDA–Ce6 nanoparticles for combined PDT and PTT, focusing on their characterization, cellular uptake, phototoxicity, and therapeutic effects.
2：Sample Selection and Data Sources:
Human colorectal cancer HCT-116 cells and BALB/c-nude mice were used for in vitro and in vivo studies, respectively.
3：List of Experimental Equipment and Materials:
Included polydopamine nanoparticles, chlorin e6, hyaluronic acid, and various chemicals for nanoparticle synthesis and characterization.
4：Experimental Procedures and Operational Workflow:
Synthesis of HA–PDA–Ce6, characterization, in vitro and in vivo evaluation of photodynamic and photothermal effects, and assessment of tumor targeting and therapeutic efficacy.
5：Data Analysis Methods:
Statistical analysis was performed using Student’s t test, one-way ANOVA followed by SNK or Dunnett-t test with significance set at p < 0.05.
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Zetasizer Nano ZS90
ZS90
Malvern
Particle size and ζ-potential measurements
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Microplate Reader
Multiskan GO
Thermo Fisher Scientific
Recording UV–Vis spectra
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Fluorescence spectrophotometer
RF-5301pc
Shimadzu
Recording fluorescence spectra
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Confocal laser scanning microscopy
FV1000
Olympus
Observation of cellular uptake and distribution
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Transmission electron microscope
JEM-1230
JP
Observation of nanoparticle morphology
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High-performance liquid chromatography
Waters 2690
Waters
Determination of Ce6 content
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Fiber optic-coupled laser system
Changchun New Industries Optoelectronics Technology Co., Ltd
Laser irradiation for photothermal and photodynamic therapy
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Infrared camera
Fotric 326
Fotric
Recording thermographs and temperature during irradiation
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Flow cytometry
BD Biosciences
Quantitative analysis of intracellular ROS and apoptosis
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