1：Experimental Design and Method Selection

The study utilized DFT and electrochemical studies to estimate the thermodynamic driving force of PeT in BODIPY-chromanol constructs. The methodology involved synthesizing a series of BODIPY-chromanol compounds with varying substituents and linker lengths to study their effect on PeT efficiency.

2：Sample Selection and Data Sources

Samples included newly prepared and previously reported fluorogenic BODIPY-chromanol constructs. Data were sourced from absorption and emission spectra, cyclic voltammograms, and fluorescence lifetime measurements.

3：List of Experimental Equipment and Materials

Equipment included a Hitachi U-2800 UV?vis?NIR spectrophotometer, PTI QuantaMaster spectrofluorimeter, Varian VNMRS 500 or Bruker AVIIIHD 500 NMR instruments, Bruker maXis impact mass spectrometer, CHI760C potentiostat, and Picoquant Fluotime 200 Time Correlated Single Photon Counting setup. Materials included 8-Acetoxymethyl-2,6-diethyl-1,3,5,7-tetramethyl-pyrromethene fluoroborate (PM605), HPLC grade solvents, and other chemicals from Sigma-Aldrich.

4：Experimental Procedures and Operational Workflow

The synthesis involved condensation and coupling reactions to prepare BODIPY-chromanol constructs. Photophysical and electrochemical parameters were measured for these constructs. Oxidation of probes with peroxyl radicals was monitored over time until fluorescence intensity reached a maximum plateau.

5：Data Analysis Methods

Data analysis involved calculating fluorescence quantum yields, lifetimes, and rate constants for PeT. DFT calculations were used to predict and visualize PeT between chromanol and BODIPY moieties.