研究目的
To evaluate the influence of AC electric field in fully saturated hydrocarbon chains as present in DLPC.
研究成果
The lipid membrane of the DLPC GUVs can be deformed by employing electric field cycles. The addition of concentrated glucose solution causes osmotic imbalance and enables the formation of filaments at the outer region of the GUVs, which retract under external electric field.
研究不足
The rapid movement of the vesicles in suspension after the addition of glucose hinders the analysis.
1：Experimental Design and Method Selection:
The study used optical fluorescence microscopy to investigate the influence of AC electric fields on GUVs.
2：Sample Selection and Data Sources:
GUVs were produced from DLPC and observed under various electric field conditions.
3：List of Experimental Equipment and Materials:
Included a cell for electric field application, an inverted microscope, and a confocal laser scanning microscope.
4：Experimental Procedures and Operational Workflow:
GUVs were subjected to electric fields of varying strengths and frequencies, and their responses were recorded.
5：Data Analysis Methods:
The images were analyzed using specific software to measure deformation and filament retraction speeds.
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1,2-dilauroyl-sn-glycero-3-phosphatidylcholine
DLPC
Avanti Polar Lipids
Used as the primary lipid component for forming giant unilamellar vesicles (GUVs).
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1,2-dioleoyl-sn-glycero-3-phosphatidylethanolamine-N-(lissamine rhodamine B sulfonyl)
rhodamine-DOPE
Avanti Polar Lipids
Fluorescent lipid used to label the GUVs for visualization under fluorescence microscopy.
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cell for the application of the electric field
Eppendorf
Used to apply electric fields to the GUVs during observation under the microscope.
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inverted microscope
TE 2000
Nikon
Used to observe the GUVs under fluorescence microscopy.
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confocal laser scanning microscopy
Nikon C1 scanner
Nikon
Used to obtain three-dimensional (3D) images of the GUVs.
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helium-neon laser
Melles-Griot
Used for excitation at 543 nm in confocal mode.
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digital camera
Diagnostic Instruments NDIAG 1800
Coupled to the microscope for acquiring images and videos.
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cryoscopic osmometer
Osmomat 030
Gonotec
Used to match the osmolarities of the sucrose and glucose solutions.
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