研究目的
To develop and validate the methodology of carbon dioxide concentration in the evaluation of vigor of Triticum aestivum L. seeds based on infrared spectroscopy.
研究成果
The study concluded that incubating Triticum aestivum seeds at 25 °C for a minimum of 12 h is effective for evaluating respiratory activity and classifying seed lots by vigor levels. The proposed method is precise and agrees with the reference method, making it suitable for CO2 measurement in seeds.
研究不足
The study focused on Triticum aestivum L. seeds, and the methodology may require adaptation for other species. The precision and accuracy of the method were evaluated under controlled conditions, and field applicability may vary.
1：Experimental Design and Method Selection:
The study used infrared spectroscopy to quantify CO2 content released by wheat seeds during respiration. A drag system and exhaustion method were employed to measure the gas released by the seeds.
2：Sample Selection and Data Sources:
Six lots of Triticum aestivum seeds (cultivar CD 123) were used, with samples of 50 seeds incubated at 15, 25, and 40 °C. CO2 content was quantified after 1, 3, 6, 9, 12, and 24 h of incubation.
3：List of Experimental Equipment and Materials:
An IRGA gas exchange meter (LI-COR 6400 XT), glass containers, deionized water, rubber septa for injectables, plastic hoses, disposable hypodermic needles, and BOD-type chambers were used.
4：Experimental Procedures and Operational Workflow:
Seeds were incubated in glass containers with deionized water to reach 30% moisture content. CO2 concentration was measured using the IRGA meter, with readings taken at specified intervals.
5：Data Analysis Methods:
Data were analyzed for normality and variance homogeneity, followed by ANOVA. Correlation coefficients between CO2 concentration and vigor tests were calculated.
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