研究目的
Investigating the therapeutic effects of a photon-responsive antibacterial nanoplatform on drug-resistant bacteria-caused skin infection.
研究成果
The photon-controlled antibacterial platform exhibits outstanding antibacterial activity by coupling the photothermal effect with pharmacological action, achieving synergistic photothermal therapy and pharmacotherapy of bacterial infections. It shows potential clinical applications in treating antibiotic-resistant bacteria-caused skin abscess.
研究不足
The study focuses on MRSA and may not cover all types of drug-resistant bacteria. The long-term stability and potential toxicity of BPQDs-vanco@liposome in clinical applications need further investigation.
1：Experimental Design and Method Selection:
The antibacterial platform was designed by encapsulating black phosphorus quantum dots (BPQDs) and vancomycin inside a thermal-sensitive liposome. The photothermal effect of BPQDs under NIR irradiation was utilized to disrupt the liposome and release the encapsulated antibiotics.
2：Sample Selection and Data Sources:
Methicillin-resistant Staphylococcus aureus (MRSA) was used as the model bacteria.
3：List of Experimental Equipment and Materials:
Transmission electron microscopy (TEM), atomic force microscopy (AFM), confocal laser scanning microscopy (CLSM), scanning electron microscope (SEM), Raman spectrometer, UV-vis absorbance spectrometer, FLIR A35 infrared thermal imaging camera, plate reader, fluorescence spectrometer.
4：Experimental Procedures and Operational Workflow:
The BPQDs-vanco@liposome was prepared and characterized. Its photothermal performance and NIR-triggered release behavior were tested. In vitro and in vivo antibacterial activities were evaluated.
5：Data Analysis Methods:
The growth kinetics of MRSA were monitored by detecting the optical density at 600 nm (OD600 nm). The number of viable colonies was counted using the standard spread plate method. Live and dead bacterial cells were stained and observed under fluorescence microscope.
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Transmission electron microscope
JEM-2100
JEOL
Used to observe the morphologies of BPQDs.
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Atomic force microscopy
Multimode 8
Bruker
Used to observe the topographic morphology of BPQDs.
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Confocal laser scanning microscope
FV1200
OLYMPUS
Used to obtain light microscopy (LM) images and confocal fluorescence images.
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UV-vis absorbance spectrometer
UV-2550
Shimadzu
Used to record the UV-vis spectra.
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Infrared thermal imaging camera
FLIR A35
FLIR
Used to monitor the NIR laser-induced heat conversion curves.
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Fluorescence spectrometer
F-4600
Hitachi. Ltd.
Used to obtain fluorescence results.
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BP crystals
Nanjing XFNANO Materials Tech Co., Ltd
Used as a photothermal reagent in the antibacterial platform.
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Vancomycin
Sigma-Aldrich
Used as an antibiotic in the antibacterial platform.
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Cholesterol
Avanti Polar Lipids, Inc.
Used in the preparation of liposomes.
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1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC)
Avanti Polar Lipids, Inc.
Used in the preparation of liposomes.
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1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy-(polyethyleneglycol)-amine] (DPPE-PEG-NH2, MW 2000)
Avanti Polar Lipids, Inc.
Used in the preparation of liposomes.
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N-methyl-2-pyrrolidone (NMP)
Sinopharm Chemical Reagent Co., Ltd., China
Used in the synthesis of BPQDs.
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Chloroform
Sinopharm Chemical Reagent Co., Ltd., China
Used in the preparation of liposomes.
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Calcein-AM
Dojindo Molecular Technologies
Used to stain live MRSA cells.
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Propidium iodide (PI)
Beyotime Institute of biotechnology
Used to stain dead MRSA cells.
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Cell Counting Kit-8 (CCK-8)
Beyotime Institute of biotechnology
Used to measure cell viability.
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Fetal bovine serum (FBS)
Thermo Fisher Scientific Inc.
Used in cell culture.
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Dulbecco's modified eagle medium (DMEM)
Thermo Fisher Scientific Inc.
Used in cell culture.
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Raman Spectrometer
RM 2000 Microscopic Confocal Raman Spectrometer
Renishaw PLC
Used to record the Raman spectra.
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Plate reader
PowerWave XS2
BioTek
Used to test the optical density.
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