研究目的
Investigating a new method for the detection of solution concentration using a paper-based micro?uidic device to decrease the consumption of the sample solution to be detected.
研究成果
A paper-based micro?uidic device can be fabricated using the presented method of hollowing technology. Colored solution concentration can be detected on the paper-based micro?uidic device with a volume of the solution only several micro-liters and a maximal error of only 2%.
研究不足
The volume of the solution using the traditional image-based method is usually several 10 ml, which should be improved. The presented method aims to decrease this volume to several micro-liters.
1：Experimental Design and Method Selection
The method involves designing and fabricating a paper-based micro?uidic device using printed circuit board technology and hollowing technology. The device is used for colorimetric detection of solution concentration.
2：Sample Selection and Data Sources
Litmus solutions with different concentrations were used as samples. The images of the detective zones were obtained using a microscope with charge-coupled device.
3：List of Experimental Equipment and Materials
Printed circuit board, copper sheet, solid paraffin, filter paper, microscope with charge-coupled device, litmus solutions.
4：Experimental Procedures and Operational Workflow
The procedure includes designing the pattern, transferring it to a printed circuit board, filling the hollowed zone with paraffin, covering with filter paper, melting the paraffin, pipetting the solution on the device, and capturing images for analysis.
5：Data Analysis Methods
The color parameters in RGBI model were calculated using MATLAB to determine the solution concentration.
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