研究目的
To design a simple and feasible sensing strategy for sensitive detection of low-abundant proteins, specifically carcinoembryonic antigen (CEA), using aptasensor-conjugated multifunctional magnetic beads coupled with dynamic light scattering technique.
研究成果
The developed DLS-based aptasensor offers a simple, enzyme-free, and low-cost method for the sensitive detection of CEA, with potential applicability for other proteins or biomolecules by controlling target antibody.
研究不足
The study does not discuss the potential interference from complex biological matrices in real-world applications or the scalability of the method for high-throughput screening.
1：Experimental Design and Method Selection:
The study employed a sandwich-type assay format for quantitative monitoring of CEA, utilizing aptamer-functionalized magnetic beads and dynamic light scattering (DLS) technique.
2：Sample Selection and Data Sources:
CEA standards with different concentrations were tested. Human serum specimens containing different-concentration CEA were also monitored.
3：List of Experimental Equipment and Materials:
Carboxylated magnetic beads (size: 50 nm, 25 mg mL-1,
4：3 × 1016 particles g-1, Chemicell GmbH), N-hydroxysuccinimide, N-(3-dimethylamino propyl)-N'-ethyl-carbodiimide hydrochloride, aptamers for CEA, TEM (H-7650, Hitachi Instruments), DLS (Malvern Zetasizer nanoapplication software), FTIR (Vector 22, Bruker). Experimental Procedures and Operational Workflow:
Aptamers were covalently conjugated to magnetic beads via carbodiimide coupling. The as-prepared Apt-MB was used for CEA detection by adding different-concentration standards into the mixture including the equivoluminal Apt1-MB and Apt2-MB.
5：Data Analysis Methods:
The change in the average hydrodynamic diameter (DH) was monitored by DLS to evaluate the concentration of target CEA.
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