研究目的
Investigating the design and synthesis of a novel fluorescence probe based on coumarin for the relay recognition of Al3+ and F? ions in aqueous solutions and living cells.
研究成果
The probe L2 demonstrates high selectivity and sensitivity for Al3+ and F? ions, with low detection limits. It successfully applies to real samples and living cells, indicating its potential for environmental and biological applications.
研究不足
The probe's performance is limited by the pH range (5.0~9.0) for optimal detection of Al3+ and F?. Strong acid or base conditions affect the detection accuracy.
1：Experimental Design and Method Selection:
The probe L2 was designed and synthesized for relay recognition of Al3+ and F?. The optical properties and detection properties were studied through UV-Vis spectra and fluorescence emission spectra.
2：Sample Selection and Data Sources:
Metal ions and anions stock solutions were prepared from corresponding salts. The probe L2 stock solution was prepared with dimethylsulfoxide and diluted with HEPES buffer.
3：List of Experimental Equipment and Materials:
Instruments included a Cary Eclipse fluorescence spectrometer and a Leica DMI4000B inverted fluorescence microscope. Materials included various salts and solvents.
4：Experimental Procedures and Operational Workflow:
Fluorescence emission spectra were measured with different metal ions and anions. The response time and pH conditions were optimized.
5：Data Analysis Methods:
The binding ratio and detection limits were calculated using Job’s plot and titration experiments. DFT calculations were performed for mechanism discussion.
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