研究目的
To develop new, sensitive, and simple spectrofluorimetric and spectrophotometric methods for the determination of troxerutin in pharmaceutical preparations.
研究成果
The developed spectrofluorimetric and spectrophotometric methods are simple, accurate, sensitive, and selective, suitable for routine quality control of troxerutin in pharmaceutical formulations. The spectrofluorimetric method is particularly sensitive, capable of measuring concentrations as low as 0.04 μg/mL.
研究不足
The study did not explore the applicability of the method to biological samples, which is mentioned as under investigation.
1：Experimental Design and Method Selection:
The study developed two methods based on the inhibitory effect of troxerutin on hemoglobin-catalyzed reaction of H2O2 with rhodamine B.
2：Sample Selection and Data Sources:
Pharmaceutical dosage forms of troxerutin were used.
3：List of Experimental Equipment and Materials:
FP-750 spectrofluorimeter, V-530 UV-visible spectrophotometer, TB-85 thermostat bath, pHS-3C precision pH meter, hemoglobin, rhodamine B, H2O2, troxerutin.
4：Experimental Procedures and Operational Workflow:
Detailed procedures for spectrofluorimetric and spectrophotometric measurements were followed, including pH adjustment, reaction conditions, and measurement of fluorescence and absorbance.
5：Data Analysis Methods:
Linear regression analysis was used to correlate percentage inhibition with troxerutin concentration.
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