1：Experimental Design and Method Selection:

The study involved inducing retinal I/R injury in adult C57BL/6J wild-type (WT) and CHOP knockout (Chop?/?) mice by raising IOP to 120 mm Hg for 60 minutes. Expression of CHOP and other UPR markers was studied by Western blot and immunohistochemistry. Retinal ganglion cell counts were performed in retinal flat mounts stained with an RGC marker. Retinal ganglion cell function was evaluated by scotopic threshold response (STR) electroretinography.

2：Sample Selection and Data Sources:

Adult wild-type (WT) C57BL/6J mice and Chop?/? (B6.129S[Cg]-Ddit3tm2.1Dron/J; stock no. 005530) breeders were obtained from Jackson Laboratory (Bar Harbor, ME, USA).

3：129S[Cg]-Ddit3tm1Dron/J; stock no. 005530) breeders were obtained from Jackson Laboratory (Bar Harbor, ME, USA). List of Experimental Equipment and Materials:

3. List of Experimental Equipment and Materials: Equipment included a handheld multispecies ElectroRetinoGraph instrument (model 2000, HMsERG unit; Ocuscience, Kansas City, MO, USA), Eclipse Ti inverted microscope (Nikon Instruments, Inc., Melville, NY, USA), and CRi Nuance FX multispectral imaging system with Nuance version

4：0 software (Caliper Life Sciences, Hopkinton, MA, USA). Materials included various antibodies for immunoblotting and immunohistochemistry. Experimental Procedures and Operational Workflow:

Retinal I/R injury was induced, and retinas were collected at various time points post-injury for analysis. Immunoblotting, immunohistochemistry, RNA isolation, qRT-PCR, retinal flat mounts, and ERG were performed.

5：Data Analysis Methods:

Statistical analysis was performed using Prism version 5.0 software (GraphPad, Inc., La Jolla, CA, USA). Data from two experimental groups were analyzed using unpaired Student t-tests. To analyze three or more experimental groups, one-way analysis of variance (ANOVA) was used, followed by a Tukey post hoc test.