研究目的
To establish a novel method for the determination of proteins based on the enhanced fluorescence intensity derived from the binding interaction of phenanthorline with proteins.
研究成果
A novel, sensitive, and accurate method for protein determination using phenanthorline was successfully established, offering advantages over traditional methods like the Coomassie Brilliant Blue assay.
研究不足
The method's sensitivity and accuracy are high, but it may be affected by the presence of certain metal ions and surfactants, requiring masking agents in some cases.
1：Experimental Design and Method Selection:
A fluorescence spectroscopy-based method was developed to study the interaction between phenanthorline and proteins.
2：Sample Selection and Data Sources:
Human serum albumin (HSA), bovine serum albumin (BSA), lysozyme (Lys), pepsin (Pep), and casein were used as protein samples.
3：List of Experimental Equipment and Materials:
An F-4500 fluorescence spectrophotometer and a PHS-3C Accurate pH meter were used. Phenanthorline and CH3COOH-CH3COONa buffer were among the reagents used.
4：Experimental Procedures and Operational Workflow:
The procedure involved mixing phenanthorline, buffer solution, and protein samples, then measuring fluorescence intensities at specific wavelengths.
5：Data Analysis Methods:
The fluorescence intensity was analyzed to determine protein concentrations, with calibration curves established for different proteins.
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