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Quantification of Total Phenolic and Carotenoid Content in Blackberries (Rubus Fructicosus L.) Using Near Infrared Spectroscopy (NIRS) and Multivariate Analysis

DOI:10.3390/molecules23123191 期刊:Molecules 出版年份:2018 更新时间:2025-09-04 15:30:14
摘要: A rapid method to quantify the total phenolic content (TPC) and total carotenoid content (TCC) in blackberries using near infrared spectroscopy (NIRS) was carried out aiming to provide reductions in analysis time and cost for the food industry. A total of 106 samples were analysed using the Folin-Ciocalteu method for TPC and a method based on Ultraviolet-Visible Spectrometer for TCC. The average contents found for TPC and TCC were 24.27 mg·g?1 dw and 8.30 μg·g?1 dw, respectively. Modified partial least squares (MPLS) regression was used for obtaining the calibration models of these compounds. The RPD (ratio of the standard deviation of the reference data to the standard error of prediction (SEP)) values from external validation for both TPC and TCC were between 1.5 < RPDp < 2.5 and RER values (ratio of the range in the reference data to SEP) were 5.92 for TPC and 8.63 for TCC. These values showed that both equations were suitable for screening purposes. MPLS loading plots showed a high contribution of sugars, chlorophyll, lipids and cellulose in the modelling of prediction equations.
作者: Eva María Toledo-Martín,María del Carmen García-García,Rafael Font,María Salinas-Navarro,Pedro Gómez,José Manuel Moreno-Rojas,Mercedes del Río-Celestino
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To study the potential of the NIRS technology for predicting the total phenolic and total carotenoid contents in blackberries, being these compounds constituting some of the main responsible molecules of the antioxidant properties in this fruit; to provide some knowledge about the mechanism used by NIRS for successfully determining these compounds in the fruits of this species.

The NIRS technique has the potential to reduce the cost and time in analysing the total phenolic and carotenoid content in blackberries for both agri-food applications and research. The spectral regions corresponding to absorbance by cellulose, lipids, chlorophyll and sugars were used by MPLS for modelling the prediction equations for total phenolic and carotenoid content in blackberries.

The models for both TPC and TCC can be classified as models that can be used for rough predictions of samples, indicating potential areas for optimization to improve predictive accuracy.

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