研究目的
To develop an ultrasensitive 'on-off' ECL biosensor for the detection of cystatin C (Cys C) in serum samples based on monolayer rubrene functionalized graphene composite (G/mRub) and immunorecognition-induced 3D DNA machine.
研究成果
The developed ECL biosensor based on G/mRub and immunorecognition-induced 3D DNA machine showed high sensitivity, wide linear range, and low limit of detection for Cys C in human serum, indicating its potential for clinical diagnosis and the preparation of high-performance luminescent nanomaterials.
研究不足
The study does not explicitly mention limitations, but potential areas for optimization could include the stability and reproducibility of the G/mRub composites and the 3D DNA machine in various clinical samples.
1：Experimental Design and Method Selection:
The study involved the synthesis of G/mRub-Pt composites via one-step re-precipitation method and the construction of an enzyme-free 3D DNA machine for signal amplification.
2：Sample Selection and Data Sources:
Human serum samples were used for the detection of Cys C.
3：List of Experimental Equipment and Materials:
Included graphene oxide, rubrene, chloroplatinic acid, gold chloride, ascorbic acid, and various DNA oligonucleotides.
4：Experimental Procedures and Operational Workflow:
The process included the preparation of G/mRub-Pt composites, assembly of 3D DNA machines, fabrication of the ECL biosensor, and ECL measurement procedure.
5：Data Analysis Methods:
The ECL intensity changes were analyzed to determine the concentration of Cys C.
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transmission electron microscope
7500
Hitachi
Used for characterizing the morphologies of nanomaterials.
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scanning electron microscopy
S-4800
Hitachi
Used for characterizing the morphologies of nanomaterials.
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atomic force microscopy
Bruker Dimension Icon
Bruker
Used for characterizing the thickness in the stepwise assembly process of G/mRub-Pt composites.
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UV-vis spectrophotometer
UV-2450
Shimadzu
Used for UV-vis absorption spectra characterization.
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fluorescence spectrophotometer
Cary Eclipse
Agilent Technologies
Used for fluorescence spectra characterization.
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graphene oxide
Xianfeng Nano Materials Tech Co. Ltd.
Used as a nanocarrier for the assembly of monolayer rubrene.
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rubrene
Sigma-Aldrich Chemical Co.
Used as the ECL emitter in the biosensor.
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chloroplatinic acid
Sigma-Aldrich Chemical Co.
Used for the in situ reduction of Pt nanoparticles on the surface of G/mRub.
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gold chloride
Sigma-Aldrich Chemical Co.
Used for the preparation of Au nanoparticles.
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ascorbic acid
Sigma-Aldrich Chemical Co.
Used as a reducing agent for the synthesis of G/mRub-Pt composites.
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electrochemistry workstation
CHI660D
Shanghai Chenhua Instruments
Used for electrochemical and ECL measurements.
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MPI-E multifunctional analyzer
MPI-E
Xi'An Remax Electronic Science & Technology Co. Ltd.
Used for ECL measurements.
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X-ray photoelectron spectroscopy
VG Scientific ESCALAB 250
VG Scientific
Used for elemental composition analysis of G/mRub-Pt composites.
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