研究目的
Investigating the therapeutic effects of a combination of doxorubicin (DOX)-based chemotherapy and zinc phthalocyanine (ZnPC)-based photodynamic therapy (PDT) using in situ-formed thermal-responsive copolymer hydrogel for bladder cancer therapy.
研究成果
The thermosensitive thermal-responsive hydrogel is a promising in situ-formed matrix for DOX- and ZnPC-based photo/chemo combination treatment for bladder cancer therapy, showing significant tumor inhibition without obvious toxicity.
研究不足
The study focuses on a specific combination therapy for bladder cancer and may not be directly applicable to other types of cancer. The in vivo studies were conducted in nude mice, which may not fully replicate human physiological conditions.
1：Experimental Design and Method Selection:
The study involved the synthesis of a tri-block copolymer PCL-PTSUO-PEG and the preparation of thermal-responsive nanoparticles (TNPs) using nanoprecipitation technology. The thermal-responsive hydrogel was formed by heating TNP solution at 37°C.
2：Sample Selection and Data Sources:
5637 cells and nude mice bearing 5637 cells xenograft were used.
3：List of Experimental Equipment and Materials:
Doxorubicin hydrochloride, 5637 cells, RPMI 1640 Medium, DAPI, MTT, Reactive Oxygen Species Assay Kit, and other chemicals were used.
4：Experimental Procedures and Operational Workflow:
The study included the preparation and characterization of copolymer and drug-loaded TNPs, preparation of thermosensitive hydrogels, in vitro drug release, cellular uptake experiment, in vitro cell killing efficiency, intracellular ROS generation, and in vivo antitumor activity.
5：Data Analysis Methods:
Data were analyzed using Student’s t-test with GraphPad Prism 6.0.
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