研究目的
To develop a simple and sensitive spectrofluorimetric method for the determination of vandetanib (VDB) in tablets and biological fluids (spiked human plasma and urine).
研究成果
The proposed spectrofluorimetric method is simple, sensitive, and can be successfully applied for the quantitation of VDB in pharmaceutical preparations and biological fluids. It offers advantages over chromatographic methods in terms of simplicity, rapidity, and cost.
研究不足
The method's sensitivity and specificity are high, but it requires optimization of factors affecting fluorescence intensity. The study did not explore the method's applicability to other biological matrices beyond plasma and urine.
1：Experimental Design and Method Selection:
The method is based on examining the intrinsic fluorescence intensity of VDB in acetonitrile at 480 nm after excitation at 330 nm. Factors affecting fluorescence intensity, including pH, diluting solvent, and time, were studied and optimized.
2：Sample Selection and Data Sources:
VDB reference standard was purchased, and human plasma was provided by King Khaled University Hospital.
3：List of Experimental Equipment and Materials:
Jasco FP-8200 Fluorescence Spectrometer, acetonitrile, methanol, ethanol, ultrapure water, and various buffers.
4：Experimental Procedures and Operational Workflow:
Preparation of standard solutions, construction of calibration graph, assay of VDB tablet samples, and determination of VDB in human plasma and urine.
5：Data Analysis Methods:
Data analysis was carried out using Microsoft Excel-2007 and Spectra Manager? software.
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