研究目的
To develop a colorimetric approach for the determination of cadmium(II) using unmodified gold nanoparticles (AuNPs) as a colorimetric probe.
研究成果
An ultrasensitive colorimetric assay for Cd2+ detection is presented. It is based on the Cd2+-GSH complex-triggered AuNP aggregation in salt media with dark-field microscope. The method does not need AuNP surface modification and complicated experimental steps.
研究不足
The sensitivity of this method needs to be further improved to the fM level. Moreover, it is hopeful to find other GSH-like molecules as complexing agents for heavy metal ions to detect other heavy metal ions.
1：Experimental Design and Method Selection:
The assay is based on AuNP aggregation induced by the complexes formed between Cd(II) and glutathione (GSH) in a concentrated solution of NaCl.
2：Sample Selection and Data Sources:
Different concentrations of Cd2+ solutions were added into the GSH solution, followed by the addition of AuNP solution and NaCl solution.
3：List of Experimental Equipment and Materials:
Gold(III) chloride trihydrate (HAuCl4·3H2O), trisodium citrate, and L-Glutathione (GSH) were used.
4：Experimental Procedures and Operational Workflow:
The mixture was incubated, and the solution was observed under dark-field microscopy.
5：Data Analysis Methods:
The intensity change of AuNPs was monitored as a ratio of absorbances at 600 and 520 nm.
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Gold(III) chloride trihydrate
HAuCl4·3H2O
Sigma-Aldrich
Used as a reagent in the synthesis of gold nanoparticles.
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L-Glutathione
GSH
Sigma-Aldrich
Used as a sensing element in the experiment to prevent AuNP aggregation in high-salt solution.
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Transmission electron microscopy
H-7650
Hitachi
Used for TEM imaging of AuNPs.
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Trisodium citrate
Sigma-Aldrich
Used as a reducing agent in the synthesis of gold nanoparticles.
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UV-2550 Spectrophotometer
Shimadzu Corporation
Used for UV-vis absorption measurements.
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Microscope
DS-Fi2
Nikon
Used for dark-field imaging of AuNPs.
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Image-Pro Plus 6.0 software
Used to process the dark-field microscopy images of AuNPs.
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