研究目的
To develop a 28-color flow cytometry panel for the in-depth characterization of B cells, dendritic cells, and monocytes in cryopreserved peripheral blood mononuclear cells (PBMC).
研究成果
The 28-color flow cytometry panel described enables comprehensive analysis of monocytes, dendritic cells, and B cells, their subsets and phenotypes in humans, and is valuable for studying alterations associated with infectious and autoimmune diseases.
研究不足
The panel may not achieve the same depth for dendritic cell analysis as some specialized panels, and some markers show limited cross-reactivity with PBMC from rhesus macaques.
