1：Experimental Design and Method Selection:

The study involved synthesizing a non-ionic amphiphilic acceptor A1 and using Pt(II) octaethylporphyrin (PtOEP) as a donor. The experimental design focused on self-assembly in water to protect against oxygen quenching and enable photon upconversion. Methods included synthesis, characterization (NMR, DLS, TEM, UV-vis, fluorescence spectroscopy), and TTA-UC emission measurements under aerated and deaerated conditions.

2：Sample Selection and Data Sources:

Samples included A1 synthesized in the lab and commercial PtOEP. Aqueous dispersions were prepared by dissolving A1 and PtOEP in CHCl3, evaporating the solvent, adding water, and sonicating. Concentrations ranged from 40 μM to 400 μM for A1 and 0.1 μM to 1 μM for PtOEP.

3：1 μM to 1 μM for PtOEP. List of Experimental Equipment and Materials:

3. List of Experimental Equipment and Materials: Equipment included JNM-ECZ400S NMR spectrometer, Malvern Nano-ZS ZEN3600 for DLS, JASCO V-770 spectrophotometer for absorption, PerkinElmer LS 55 fluorescence spectrometer, HAMAMATSU multichannel analyzer C10027-01 for quantum yield, time-correlated single photon counting systems (C11367-02 and C11567-01) for lifetime measurements, diode laser (532 nm, 200 mW, RGB Photonics) for excitation, PD300-UV photodiode sensor (OPHIR Photonics) for power measurement, CCD beam profiler SP620 (OPHIR Photonics), multichannel detector MCPD-9800 (Otsuka Electronics), and absolute quantum yield measurement system C13534-01 (Hamamatsu Photonics). Materials included CHCl3, D2O, CDCl3, Milli-Q water, and various chemicals for synthesis.

4：Experimental Procedures and Operational Workflow:

A1 was synthesized and characterized. Aqueous dispersions were prepared by sonication. DLS and TEM were used to confirm assembly formation. Absorption and fluorescence spectra were recorded. For TTA-UC, samples were excited with a 532 nm laser, and emission was measured with notch filters to remove excitation light. Deaeration was done by freeze-pump-thaw cycles. Excitation intensity dependence and lifetime measurements were conducted.

5：Data Analysis Methods:

Data analysis included estimating assembly sizes from DLS, spectral analysis from UV-vis and fluorescence, calculation of quantum yields using integrating spheres, and fitting decay curves for triplet lifetimes. Excitation intensity plots were used to confirm TTA mechanism.