研究目的
To develop cationic carbon dots (cCDs) for application in fingerprinting analysis, offering a biocompatible and rapid alternative to traditional methods in forensic science.
研究成果
The cCDs are effective for rapid and detailed fingerprint visualization on multiple surfaces, with multi-fluorescence properties enabling enhanced analysis. They are biocompatible and suitable for forensic applications, potentially leading to handheld detection devices.
研究不足
The study is limited to laboratory conditions; real-world forensic applications may involve more complex surfaces and environmental factors. The synthesis requires optimization for scale-up, and long-term stability of cCDs on various surfaces was not extensively tested.
1：Experimental Design and Method Selection:
One-pot microwave-assisted synthesis of cCDs using citric acid and polyethyleneimine (PEI) as precursors. Characterization includes UV-Vis spectroscopy, photoluminescence, TEM, XRD, Raman, FTIR, XPS, and confocal microscopy for fingerprint analysis.
2：Sample Selection and Data Sources:
cCDs synthesized and characterized; fingerprints from volunteers on various surfaces (e.g., glass, plastic, currency).
3：List of Experimental Equipment and Materials:
Microwave oven (180W), centrifuge, Millipore filter (0.22 μm), dialysis tubing, lyophilizer, UV lamp (375 nm), cell phone camera, confocal laser scanning microscope (CLSM), flow cytometer for ROS analysis. Materials include citric acid, PEI, PBS, phosphatidyl choline, DCFDA.
4：22 μm), dialysis tubing, lyophilizer, UV lamp (375 nm), cell phone camera, confocal laser scanning microscope (CLSM), flow cytometer for ROS analysis. Materials include citric acid, PEI, PBS, phosphatidyl choline, DCFDA.
Experimental Procedures and Operational Workflow:
4. Experimental Procedures and Operational Workflow: Synthesis involves mixing citric acid and PEI in PBS, microwave heating, centrifugation, filtration, dialysis, and lyophilization. Fingerprint development by applying cCDs solution to surfaces, imaging under UV light with cell phone or microscope.
5：Data Analysis Methods:
Image analysis using ImageJ software for size distribution and intensity profiles; spectroscopic data analysis for quantum yield, band gap, and chemical composition.
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