1：Experimental Design and Method Selection:

The study involved synthesizing CIS heterostructured nanorods via thermal decomposition, coating with hyaluronic acid for solubility and targeting, and conjugating with chlorin e6 for photodynamic therapy. Methods included ultrasonic-assisted encapsulation and covalent coupling using cross-linkers.

2：Sample Selection and Data Sources:

B16F1 mouse melanoma cells and HeLung normal cells were used for in vitro studies; zebrafish embryos (Danio rerio AB line) were used for in vivo biocompatibility evaluation.

3：List of Experimental Equipment and Materials:

Equipment included spectrophotometers, spectrofluorometers, TEM, HRTEM, ICP-AES, DLS instrument, NIR lasers, digital thermometer, confocal microscope, microplate reader, and ultrasonic probe. Materials included copper acetate, indium acetate, 1-dodecanethiol, oleic acid, hyaluronic acid, chlorin e6, EDC, sulfo-NHS, and various buffers and reagents.

4：Experimental Procedures and Operational Workflow:

Synthesis of CIS HS-rod, HA coating, Ce6 conjugation, characterization (absorption, fluorescence, TEM, DLS), photothermal performance testing, photostability assessment, singlet oxygen measurement, cytotoxicity assays (WST-1), cellular uptake studies, in vivo zebrafish experiments, intracellular ROS measurement, and PDT/PTT treatments with laser irradiations.

5：Data Analysis Methods:

Statistical analysis using t-test with p < 0.05 considered significant; data from spectrophotometry, fluorometry, and microscopy were analyzed for trends and efficacy.