研究目的
To develop a novel sensor for the determination of acid phosphatase (ACP), a prostate cancer biomarker, using a copper phthalocyanine-modified screen-printed gold transducer, enabling sensitive and selective detection in nanomolar quantities.
研究成果
The developed sensor based on Cu(II)TC Pc-PAA modified gold electrode enables sensitive and selective detection of acid phosphatase with a linear range of 0.5-20 nM and LOD of 0.5 nM, suitable for clinical applications in prostate cancer biomarker detection. The dual detection of phosphate and naphthol enhances reliability, and good recovery in spiked samples demonstrates potential for real-world use, though further studies in clinical matrices are recommended.
研究不足
The study was conducted in controlled laboratory conditions using spiked samples; real clinical samples may have additional interferences. The sensor's performance in complex matrices like human serum needs further validation. The electrochemical method may be affected by electrode fouling or stability over long-term use.
1：Experimental Design and Method Selection:
The study involved synthesizing a copper phthalocyanine derivative (Cu(II)TC Pc-PAA) and immobilizing it on a screen-printed gold electrode for electrochemical detection of phosphate and naphthol, which are products of the enzymatic reaction of acid phosphatase with 2-naphthyl phosphate. Cyclic voltammetry was used for characterization and detection.
2：Sample Selection and Data Sources:
Acid phosphatase from wheat germ, 2-naphthyl phosphate sodium salt, and other chemicals were purchased from Sigma-Aldrich. Spiked bovine serum albumin samples were used for validation.
3：List of Experimental Equipment and Materials:
Scanning electron microscopy (JEOL JSM-6390LV model), electrochemical workstation (CH Instruments Inc. 920), screen-printed electrodes (SPE-AT-220 from DropSens), and various chemicals including Tris-HCl buffer, tetrahydrofuran, dimethylsulfoxide, etc.
4：Experimental Procedures and Operational Workflow:
The electrode was modified by drop-casting Cu(II)TC Pc-PAA solution, dried for 24 hours. Electrochemical measurements were performed in a three-electrode cell with TRIS-HCl buffer. Optimization of pH, buffer type, and incubation time was conducted. Enzyme assays involved incubating ACP with substrate and measuring the electrochemical response.
5：Data Analysis Methods:
Data were analyzed using cyclic voltammetry, with calibration curves constructed for phosphate and naphthol concentrations. Limits of detection were calculated based on 3σ/slope method, and reproducibility was assessed with coefficients of variation.
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