研究目的
To explore the effect of Dan Hong Injection (DHI) on the unbound warfarin using in vivo pharmacokinetic and in vitro fluorescence studies.
研究成果
DHI increases the concentration of unbound warfarin in blood due to competition for binding with serum albumin, potentially enhancing anticoagulant effects and requiring careful dosage monitoring in clinical practice.
研究不足
The study is limited to rat models and in vitro conditions; further studies are needed to confirm findings in humans and clarify mechanisms.
1：Experimental Design and Method Selection:
The study used a microdialysis sampling method coupled with LC-MS/MS for in vivo pharmacokinetic analysis and fluorescence spectrometry for in vitro binding studies.
2：Sample Selection and Data Sources:
Male Sprague-Dawley rats were used for in vivo experiments; bovine serum albumin (BSA), salvianolic acid B (SaB), and warfarin were used for in vitro studies.
3：List of Experimental Equipment and Materials:
LC-MS/MS system (Agilent 6430 Quad LC-MS/MS and Agilent 1200 model), microdialysis system (CMA), microplate reader (Perkin-Elmer), and various chemicals and reagents.
4：Experimental Procedures and Operational Workflow:
Rats were anesthetized, microdialysis probes implanted, and samples collected every 20 min for 12 h after drug administration. In vitro fluorescence experiments involved quenching studies with BSA, DHI, and SaB.
5：Data Analysis Methods:
Pharmacokinetic data analyzed using DAS 1.0 software; fluorescence data analyzed using Stern-Volmer equations.
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LC-MS/MS system
Agilent 6430 Quad LC-MS/MS
Agilent
Used for detecting the concentration of warfarin in microdialysates.
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Microplate reader
Multifunctional microplate reader
Perkin-Elmer
Used for recording fluorometric experiments.
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Chromatography column
Agilent Zorbax Eclipse Plus C18
Agilent
Used for chromatographic separation in LC-MS/MS.
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Microdialysis system
CMA/402 syringe pump-Dual syringe perfusions
CMA
Used for sampling blood microdialysates in vivo.
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Water purification system
Milli-Q system
Millipore
Used for purifying water for experiments.
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