1：Experimental Design and Method Selection:

The study involved the synthesis of BODIPY derivatives using chemical reactions such as esterification and Knoevenagel condensation, followed by characterization via NMR, HRMS, and X-ray crystallography. Photophysical properties were measured using UV-Vis and fluorescence spectroscopy, and cell imaging was performed using confocal microscopy.

2：Sample Selection and Data Sources:

HeLa cells were used as the model cell line for imaging studies, cultured in DMEM medium with fetal bovine serum.

3：List of Experimental Equipment and Materials:

Chemicals and solvents were of commercial quality, including specific reagents like SOCl2, DMF, pyridine, THF, TFA, TEA, BF3?OEt2, piperidine, PTSA, and various solvents. Equipment included UV-Vis spectrophotometers, fluorescence spectrometers, NMR spectrometers, HRMS instruments, confocal microscopes, and X-ray diffractometers.

4：Experimental Procedures and Operational Workflow:

Synthesis involved multiple steps: preparation of bornyl 4-formylbenzoate, reaction with pyrroles, and condensation reactions. Purification was done via column chromatography. For cell imaging, HeLa cells were incubated with compounds, stained with LysoTracker Green and Hoechst 33342, and imaged using confocal microscopy with specific laser excitations and emission collections.

5：Data Analysis Methods:

Photophysical data were analyzed using equations for quantum yield and rate constants. Fluorescence lifetimes were measured using time-resolved spectroscopy. Cell imaging data were analyzed through co-localization studies and Z-depth scanning.