研究目的
To develop a method for preoperative determination of intrarenal arteries using micro-CT and injection materials to create a 3D vascular tree model without removing soft tissue, supporting minimally invasive renal surgeries.
研究成果
The selected two-component epoxy standard glue with xylene and 10% iodine effectively filled vascular beds, allowing micro-CT imaging without tissue removal. This approach enables 3D reconstruction of intrarenal vascular trees, supporting preoperative planning for kidney surgeries. Future work includes optimizing the filling technique and applying it to human tissue.
研究不足
Some vascular parts had air bubbles or gaps, possibly due to internal flow factors or solidification issues. The technique may require optimization for repeatability and application to human tissue.
1:Experimental Design and Method Selection:
The study evaluated various injection materials for filling vascular beds in post-mortem kidneys to enhance micro-CT imaging. Materials were selected based on availability and affordability, and compared for properties like radiation absorption, viscosity, and solidification rate. The technique involved injecting materials into renal arteries and scanning with micro-CT.
2:Sample Selection and Data Sources:
Pork kidneys obtained on the day of slaughter were used as samples. Five kidneys were utilized for micro-CT examinations.
3:List of Experimental Equipment and Materials:
Equipment included a micro-CT device (nanotom 180N by GE), rotational viscometer (FungiLab), laboratory scale, and software (datosX, VGStudio Max, VolView). Materials tested included satin enamel, latex paint, acrylic enamel, silicone adhesive, two-component epoxy glues, barium sulfate, and iodine as a contrast agent.
4:Experimental Procedures and Operational Workflow:
Materials were prepared with solvents and contrast agents, placed in capillary containers or kidney holes, and scanned with micro-CT. Vessels were filled using a selected epoxy glue mixture injected via a needle, followed by ligation and micro-CT scanning.
5:Data Analysis Methods:
Qualitative observations and quantitative measurements of radiation absorption were performed using software tools. Data were analyzed for homogeneity, enhancement relative to kidney tissue, and 3D reconstruction of vascular trees.
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