研究目的
Development of a sensitive and selective electrochemiluminescent sensor for the detection of hydrogen sulfide (H2S) using a new co-reactant NBD-amine to enhance ECL intensity.
研究成果
The developed ECL sensor based on RuSi NPs/Nafion and NBD-amine co-reactant provides a highly sensitive and selective method for H2S detection with a low detection limit of 1.7 × 10^-12 mol/L and good performance in human serum samples, indicating potential for practical applications in biological sensing.
研究不足
The sensor requires a reaction time of 3 hours for H2S detection, which may be slow for real-time applications; it relies on specific chemical reactions that could be affected by complex biological matrices; optimization of conditions such as concentration and time is necessary for best performance.
1：Experimental Design and Method Selection:
The study employs electrochemiluminescence (ECL) with Ru(bpy)3 2+-doped silica nanoparticles (RuSi NPs) as luminophores and Nafion for immobilization on electrodes. A new co-reactant, NBD-amine, is synthesized and used to enhance ECL signals upon reaction with H2S, releasing a secondary amine that participates in electron transfer processes.
2：Sample Selection and Data Sources:
H2S is detected in PBS buffer and human serum samples, with Na2S used as an H2S source.
3：List of Experimental Equipment and Materials:
Includes glassy carbon electrode (GCE), saturated calomel electrode (SCE), platinum wire electrode, Ru(bpy)3 2+, Triton X-100, cyclohexane, 1-hexanol, TEOS, NH3·H2O, acetone, ethanol, Nafion (5 wt%), NBD-Cl, N-Boc-piperazine, PBS buffer, and human serum.
4：Experimental Procedures and Operational Workflow:
Synthesis of NBD-amine via reaction of N-Boc-piperazine with NBD-Cl; synthesis of RuSi NPs using W/O microemulsion method with Triton X-100, cyclohexane, 1-hexanol, Ru(bpy)3 2+, TEOS, and NH3·H2O, followed by centrifugation and washing; electrode preparation by polishing GCE, mixing RuSi NPs with Nafion, and drop-casting onto GCE; ECL measurements in PBS with NBD-amine and H2S, using a three-electrode system with scan potential from
5：2 to 1 V. Data Analysis Methods:
ECL intensity is measured and correlated with H2S concentration using linear regression; detection limit calculated as 3SB/m; selectivity tested against interfering species; stability and reproducibility assessed via cyclic scans and multiple sensors; recovery studies in serum samples using standard addition method.
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Glassy Carbon Electrode
GCE
Used as the working electrode in the three-electrode system for ECL measurements.
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Saturated Calomel Electrode
SCE
Used as the reference electrode in the three-electrode system.
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Platinum Wire
Pt
Used as the auxiliary electrode in the three-electrode system.
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Nafion
5 wt%
Used to immobilize RuSi NPs on the electrode surface.
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Triton X-100
Used in the microemulsion method for synthesizing RuSi NPs.
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Transmission Electron Microscope
TEM
Used to characterize the morphology and microstructure of RuSi NPs.
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Scanning Electron Microscope
SEM
Used to characterize RuSi NPs.
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UV-Vis Spectrophotometer
Used for UV-vis absorption analysis of NBD-amine with H2S.
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Fluorescence Spectrophotometer
Used for fluorescence analysis of NBD-amine with H2S.
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