研究目的
To examine self-compatibility in apricot cultivars using fluorescence microscopy, which is important for apricot breeders and producers in selecting cultivars for controlled crosses and choosing adequate pollinizers.
研究成果
Fluorescence microscopy is an effective method for determining self-incompatibility in apricots. About half of the 123 cultivars studied were self-incompatible, particularly those from new breeding programs. Self-incompatibility is undesirable for orchard production as it requires cross-pollination, so self-compatibility should be a key objective in breeding to ensure higher and more regular yields.
研究不足
The method requires well-developed one-year-old shoots; using thin or short shoots may lead to pistil drop and unusable samples. Weather conditions can affect field-based methods, but this lab-based approach mitigates that. The study is limited to the cultivars available in the collection and may not represent all apricot varieties.
1：Experimental Design and Method Selection:
The study used fluorescence microscopy to observe pollen tube growth in pistils after self-pollination, based on established methods for determining self-incompatibility in fruit-tree species.
2：Sample Selection and Data Sources:
Plant material was taken from 123 apricot cultivars in the collection at the Faculty of Agriculture in Belgrade, with origins from various countries.
3：List of Experimental Equipment and Materials:
Equipment included a Leica DM LS fluorescence microscope with filters A (340-380 nm) or I3 (450-490 nm). Materials included flower buds, sucrose solution, formaldehyde, glacial acetic acid, ethanol, NaOH, aniline blue, and K3PO
4：Experimental Procedures and Operational Workflow:
One-year-old shoots with flower buds were collected, emasculated, hand-pollinated after 24 hours, fixed after 96 hours in a formaldehyde-acetic acid-ethanol mixture, rinsed, softened in NaOH, stained with aniline blue, and examined under the microscope. The number of pollen tubes reaching the base of the style and ovule was recorded.
5：Data Analysis Methods:
The percentage of pistils with at least one pollen tube reaching specific parts was calculated to classify cultivars as self-compatible or self-incompatible.
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