研究目的
To develop a novel Fe foil-guided in-situ etching strategy for fabricating uniform Ag@AgX nanowires and apply them for sensitive photoelectrochemical detection of Leukemia DNA.
研究成果
The Fe foil-guided method successfully produces uniform Ag@AgX nanowires, enabling highly sensitive and selective PEC detection of Leukemia DNA with a low detection limit and wide linear range, showing potential for disease diagnosis.
研究不足
The method requires long reaction times (60-72 hours) for Ag@AgX formation. Ag@AgI NWs could not be formed due to reaction issues with I-. The platform may have limitations in real-world clinical applications due to complexity and potential interference.
1：Experimental Design and Method Selection:
The study uses a Fe foil-guided in-situ etching method to synthesize Ag@AgX nanowires, based on redox potential and Ksp value considerations. Photoelectrochemical detection is employed for DNA sensing.
2：Sample Selection and Data Sources:
Ag nanowires are synthesized via a polyol reduction process. Leukemia DNA sequences are used as targets, with specific sequences detailed in supporting information.
3：List of Experimental Equipment and Materials:
Includes Fe foil, ethylene glycol, polyvinylpyrrolidone (PVP), AgNO3, NaCl, NaBr, glassy carbon electrode (GCE), alumina slurries, N,N-dimethylformamide, phosphate buffer solution (PBS), gallic acid (GA), DNA sequences, tris(2-carboxyethyl)-phosphine (TCEP), mercaptoethanol (MCH), Zn2+ solution, 8-17 DNAzyme. Equipment involves oil bath, magnetic stirrer, SEM, TEM, HRTEM, EDX, XRD, XPS, EIS, and photoelectrochemical measurement setup.
4：Experimental Procedures and Operational Workflow:
Ag NWs are synthesized, then etched with anions using Fe foil to form Ag@AgX NWs. Electrodes are modified with these NWs. PEC measurements are conducted under visible light at 0 V applied potential. DNA assay involves immobilization of hairpin DNA on electrode, hybridization with target DNA, cleavage by DNAzyme, and PEC detection.
5：Data Analysis Methods:
Data is analyzed using techniques like SEM, TEM, XRD, XPS for characterization, and photocurrent responses are measured and plotted for calibration curves. Statistical analysis includes standard deviation (n=3).
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Fe foil
Used to guide the uniform growth of AgX nanoparticles on Ag nanowires by in-situ etching.
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Glassy Carbon Electrode
GCE
Serves as the base electrode for modifying with Ag@AgX nanowires and conducting photoelectrochemical measurements.
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Scanning Electron Microscope
SEM
Used to characterize the morphology of nanomaterials such as Ag NWs and Ag@AgX NWs.
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Transmission Electron Microscope
TEM
Used for high-resolution imaging to observe the core-shell structure of Ag@AgX nanowires.
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X-ray Diffractometer
XRD
Used to analyze the crystal structures of the synthesized nanomaterials.
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X-ray Photoelectron Spectrometer
XPS
Used to characterize the composition and chemical states of elements in the nanomaterials.
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Energy Dispersive X-ray Spectrometer
EDX
Used to analyze the elemental composition of the nanocomposites.
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Electrochemical Impedance Spectroscope
EIS
Used to measure the electron transfer resistance of the modified electrodes.
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