研究目的
To develop a multi-functional platform combining macroscopic scaled super-crystal morphology and multiple application properties by fabricating stripe patterned structures of gold nanorod arrays with controlled orientations for sensing enhancement and cell alignment.
研究成果
The study successfully demonstrates the fabrication of millimeter-scale stripe patterns of GNRs with controlled orientation, showing orientational dependence in SERS and electrical sensing applications. Vertically arranged GNRs enhance SERS signals, while horizontally arranged ones have higher electrical conductivity. Additionally, the patterns effectively align mammalian cells, offering potential for tissue engineering and biosensing. The method provides a versatile platform for multifunctional applications but requires careful control of assembly parameters.
研究不足
The assembly process is sensitive to parameters like aspect ratio, concentration, humidity, and surface chemistry, which may limit reproducibility. The technique may not be easily scalable to more complex patterns or other nanomaterials without optimization.
1：Experimental Design and Method Selection:
The study uses a combination of soft lithography and evaporative self-assembly to fabricate stripe patterns of gold nanorods (GNRs) on substrates, with controlled orientation for applications in sensing and cell alignment. Theoretical models involve plasmonic coupling and electrostatic interactions.
2：Sample Selection and Data Sources:
GNRs are synthesized with different aspect ratios (2.0, 2.7, 3.5, 4.5) using a seed-mediated growth method. Substrates include ITO glass and general glass slides. NIH-3T3 fibroblast cells are used for cell alignment studies.
3：0, 7, 5, 5) using a seed-mediated growth method. Substrates include ITO glass and general glass slides. NIH-3T3 fibroblast cells are used for cell alignment studies.
List of Experimental Equipment and Materials:
3. List of Experimental Equipment and Materials: Equipment includes SEM (FEI FP 2031/12 Inspect F50), UV-Vis spectrophotometer (Varioscan Lux), zeta potential analyzer (Malvern zetasizer series ZSP), SERS system (Renishaw InVia Reflex), electrical measurement instrument (Agilent Technologies B1500A), optical microscope (Olympus inverted microscope). Materials include HAuCl4, CTAB, NaBH4, NaOL, PDMS, thiol chemicals (e.g., 6-MHA, 16-MHDA, cystamine), ITO glass, silicon wafer, SU-8 resin.
4：Experimental Procedures and Operational Workflow:
Fabricate PDMS soft templates from silicon wafer masters using photolithography. Synthesize GNRs and modify surface chemistry. Assemble GNRs on substrates by pipetting suspension into channels formed between PDMS mold and substrate, allowing slow evaporation. Characterize using SEM, UV-Vis, zeta potential. Perform SERS and electrical measurements. Seed cells on patterned substrates and observe adhesion and alignment.
5：Data Analysis Methods:
Analyze SEM images for assembly morphology, UV-Vis spectra for GNRs characterization, zeta potential for surface charge. SERS intensity is measured and compared. Electrical conductivity is calculated from I-V curves. Cell aspect ratio is measured from microscopy images.
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