研究目的
To develop a modified Linnik microinterferometer for registering hyperspectral holograms of microobjects in reflection using polychromatic light, enabling quantitative phase microscopy and spectral analysis for improved hematological diagnostics.
研究成果
The modified Linnik interferometer successfully records hyperspectral holograms, allowing quantitative phase analysis and spectral profiling of RBCs, which enhances multiparametric differentiation for hematological diagnostics.
研究不足
The method may be limited by the spectral range (450-650 nm) and resolution, potential noise in narrow-band images, and the complexity of setup tuning and alignment.
1：Experimental Design and Method Selection:
A modified Linnik interferometer is used with a voice coil in the reference arm for mirror displacement to register interferograms in incoherent light, followed by Fourier transform processing to obtain hyperspectral holograms.
2：Sample Selection and Data Sources:
Human red blood cells (RBCs) fixed on a slide are used as the sample.
3：List of Experimental Equipment and Materials:
Linnik microinterferometer with microobjectives, voice coil transducer, high-speed CMOS camera (2048x2560 pixels), microscrew for zeroing optical path difference, rotation mechanism, springs, amplifier with voltage and current stabilization, polychromatic light source.
4：Experimental Procedures and Operational Workflow:
The interferometer is tuned to achieve interference fringes; the voice coil moves the mirror axially over 100 μm at 5 μm/s; interferograms are registered at 200 fps; 1D FFT is applied to obtain complex amplitudes for spectral components.
5：Data Analysis Methods:
Fourier transform spectroscopy is used to derive amplitude and phase images; denoising, phase unwrapping, and stacking of spectral images are performed to reduce noise and obtain integral phase profiles.
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